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Columns & accessories

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Categories starting with H

Items starting with H

Hydrophilic Interaction - Tosoh Bioscience

Hydrophilic Interaction - Tosoh Bioscience

Normal phase and hydrophilic interaction liquid chromatography (HILIC) are primarily used to separate polar and hydrophilic compounds. In reversed phase mode very polar compounds are often not sufficiently retained in low percent organic, or even in 100% aqueous mobile phase. The order of elution in normal phase is opposite that found in reversed phase for the same mixture of compounds. Although non-polar organic mobile phases and a silica stationary phase were used traditionally in normal phase LC, today most separations are performed with aqueous-organic mobile phases and a more polar-bonded stationary phase. This mode of HPLC is now commonly referred to as HILIC, hydrophilic interaction liquid chromatography.

Hydrophobic Interaction - Tosoh Bioscience

Hydrophobic Interaction - Tosoh Bioscience

Hydrophobic interaction chromatography (HIC) is based on non-polar interactions that are induced by high salt mobile phases. Stationary phases are similar to reversed phase chromatography (RPC) but the density of functional groups is lower. A weakly non-polar stationary phase is used with an aqueous mobile phase containing a high concentration of a chaotropic salt.

HPLC columns - Thermo Scientific

HPLC columns - Thermo Scientific

See the selection of HPLC columns from Thermo Scientific.

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