eng

  • Slide1
  • Slide2
  • Slide3

Kolonner og Tilbehør

a b c d e f g h i j k l m n o p q r s t u v w x y z #

HPLC-kolonner fra Tosoh Bioscience

 tosohlogo

 HPLC columns kolonner Tosoh

Som autoriseret forhandler kan MD Scientific tilbyde Tosoh Biosciences omfattende serie af kolonner til enhver type oprensning.

For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: Denne e-mail adresse bliver beskyttet mod spambots. Du skal have JavaScript aktiveret for at vise den. eller telefon: 7027 8565.


High Performance Liquid Chromatography

High Performance Liquid Chromatography (HPLC) and Ultra High Performance Liquid Chromatography (UHPLC) use liquid mobile phases to transport the sample components through a column packed with a solid material; the stationary phase. The "mode" of chromatography is usually defined by a combination of a certain stationary phase type with a certain mobile phase type. In order to generate a chromatogram that is suitable for qualitative or quantitative purposes, bonded phase chemistry, column dimensions, and particle and pore sizes are parameters that need to be optimized.

TSKgel Chromatography Columns

Tosoh Bioscience offers a comprehensive line of TSKgel (U)HPLC columns for use with conventional and UHPLC systems to meet your purification needs. The TSKgel product line include columns in nearly every mode of HPLC, including Size Exclusion, Ion Exchange, Reversed Phase, Hydrophobic Interaction, Affinity, and Normal Phase/HILIC.

Click on each mode listed below for a short tutorial on the mode, including an animated video, as well as the variety of TSKgel columns offered in that mode.

 Chromatography mode     Types of Compounds analyzed 
Size Exclusion Chromatography (SEC)    Natural (biopolymers), synthetic polymers
Ion Exchange Chromatography (IEC)    Charged compounds
Reversed Phase Chromatography (RPC)    Relatively hydrophobic compounds,
neutrals, weak acids, weak bases
Hydrophobic Interaction Chromatography (HIC)   Hydrophobic biomolecules
Hydrophilic Interaction Chromatography (HILIC)   Highly polar compounds
Affinity Chromatography(AFC)    Carbohydrates, proteins, specific anitgens 

MD Scientific er autoriseret forhandler i Danmark for Tosoh.

Reversed Phase - Tosoh Bioscience

Reversed Phase - Tosoh Bioscience

Reversed Phase Liquid Chromatography (RPC) has become the standard technique to analyze small molecular weight compounds. More recently, RPC has become an accepted tool for the separation of peptides, proteins and other biopolymers in the pharmaceutical, chemical and biochemical industries.

RPC separates molecules based on nonpolar interactions and requires a nonpolar stationary phase and a polar mobile phase. Typically the mobile phase consists of a mixture of water (buffer) and acetonitrile, methanol, THF, or 2-propanol.

Hydrophilic Interaction - Tosoh Bioscience

Hydrophilic Interaction - Tosoh Bioscience

Normal phase and hydrophilic interaction liquid chromatography (HILIC) are primarily used to separate polar and hydrophilic compounds. In reversed phase mode very polar compounds are often not sufficiently retained in low percent organic, or even in 100% aqueous mobile phase. The order of elution in normal phase is opposite that found in reversed phase for the same mixture of compounds. Although non-polar organic mobile phases and a silica stationary phase were used traditionally in normal phase LC, today most separations are performed with aqueous-organic mobile phases and a more polar-bonded stationary phase. This mode of HPLC is now commonly referred to as HILIC, hydrophilic interaction liquid chromatography.

Affinity - Tosoh Bioscience

Affinity - Tosoh Bioscience

Affinity Chromatography (AFC) offers the greatest potential specificity and selectivity for the isolation or purification of biomolecules. Almost all biological molecules can be purified on the basis of a specific interaction between their chemical or biological structure and a suitable affinity ligand.

Hydrophobic Interaction - Tosoh Bioscience

Hydrophobic Interaction - Tosoh Bioscience

TSKgel HIC packings consist of polymethacrylate base material and a choice of three ligands (butyl, ether and phenyl) with varied hydrophobicities from low to high, respectively.

HIC is used in the biopharmaceutical industry for the analysis of antibody drug conjugates (ADCs) or to determine the aggregate content of monoclonal antibodies.

Ion Exchange - Tosoh Bioscience

Ion Exchange - Tosoh Bioscience

Separation in Ion Exchange chromatography (IEC or IEX) is based on reversible adsorption of charged solute molecules to immobilized functional groups of opposite charge. Biomolecules generally have charged groups on their surfaces, which change with the buffer pH. Elution can be accomplished by changing the ionic strength or the pH, of which changing the ionic strength by increasing the salt concentration is most common.

Size Exclusion - Tosoh Bioscience

Size Exclusion - Tosoh Bioscience

Size Exclusion Chromatography (SEC) separates molecules based on their size, or more precisely, their hydrodynamic volume. It is based on the discrimination of individual sample components by the pores of the packing material. Large sample molecules cannot or can only partially penetrate the pores and elute from the column first, whereas smaller molecules can access all or a larger number of pores and elute later. SEC is the only mode of chromatography that does not involve interaction with a stationary phase by means of adsorption or partitioning of the solutes.

Protein A (Affinity) - Tosoh Bioscience

Protein A (Affinity) - Tosoh Bioscience

The TSKgel Protein A-5PW column has been designed for the rapid separation and robust quantification of a variety of antibodies. Monoclonal antibodies from harvested cell culture media can be captured and accurately quantitated in less than 2 minutes per injection. The column can be used for more than 2,000 injections without regeneration or cleaning.

  • Logo1
  • Logo10
  • Logo11
  • Logo12
  • Logo13
  • Logo14
  • Logo2
  • Logo3
  • Logo4
  • Logo5
  • Logo6
  • Logo7
  • Logo8
  • Logo9

  eng