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Light scattering instrumenter

Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). The dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes.

Wyatt also offers unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.


For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: Denne e-mail adresse bliver beskyttet mod spambots. Du skal have JavaScript aktiveret for at vise den. eller telefon: 7027 8565.

MD Scientific er autoriseret forhandler for Wyatt i Danmark, Norge og Sverige.

 

MALS - Multi-angle light scattering detektor MALS - Multi-angle light scattering detektor

MALS - Multi-angle light scattering detektor

MALS

Couple a DAWN®, miniDAWN® or μDAWN® multi-angle light scattering (MALS) detector to SEC or FFF for reliable characterization of distributions of molar mass / molecular weight, size, conjugation and conformation of macromolecules, aggregates, fragments, conjugates, colloids, and nanoparticles.

All measurements take place in solution, and are independent of inherently unreliable column calibration.

DLS & ELS - dynamic light scattering and electrophoretic light scattering DLS & ELS - dynamic light scattering and electrophoretic light scattering

DLS & ELS - dynamic light scattering and electrophoretic light scattering

Dynamic light scattering (DLS) measures the hydrodynamic radius of proteins, aggregates, nanoparticles, vesicles, and more. The NanoStar® uses standard microcuvette format and the DynaPro® Plate Reader II high-throughput microwell plates.

The Möbiuζ® measures electrophoretic light scattering (ELS) and DLS simultaneously to determine zeta potential or molecular charge, automated by an autosampler or manually, under a wide range of solvents.

Refraktometer - Optilab differential refractive index (dRI) detektorer Refraktometer - Optilab differential refractive index (dRI) detektorer

Refraktometer - Optilab differential refractive index (dRI) detektorer

Optilab differential refractive index (dRI) detectors are utilized primarily for on-line measurement of analyte concentration in SEC-MALS and FFF-MALS, and for determination of analyte dn/dc (refractive index increment), and the absolute refractive index (aRI) of solvents.

Optilab® dRI detectors serve as universal concentration detectors since no chromaphore or fluorophore is necessary. Required for Conjugation Analysis.

Viskometre - on-line differential viscometer Viskometre - on-line differential viscometer

Viskometre - on-line differential viscometer

The ViscoStar® III on-line differential viscometer measures the intrinsic viscosity of polymers downstream of SEC. Differential viscometry is best combined with MALS and RI for analysis of polymer conformation and Mark-Houwink-Sakurada parameters.

On its own, the ViscoStar can determination hydrodynamic radius, or may be utilized with the Universal Calibration technique to assess polymer molar mass / molecular weight over a wide range.

Field-flow fractionation (FFF) Field-flow fractionation (FFF)

Field-flow fractionation (FFF)

Eclipse® devices carry out field-flow fractionation (FFF) of macromolecules and nanoparticles ranging in size from 1 nm - 1000 nm, with no stationary phase, eliminating shear and non-ideal column packing interactions. Integrates with top-quality HPLC pumps and autosamplers.

A wide choice of flat and hollow-fiber channels separates both soluble and colloidal components, from nanogram to milligram quantities.

CG-MALS CG-MALS

CG-MALS

The Calypso® II automated composition-gradient accessory and software are used in conjunction with a MALS detector (CG-MALS) to characterize biomolecular interactions, label-free and immobilization-free. CG-MALS determines equilibrium dissociation constants self- and hetero-association as well as more complex interactions.

Also useful for automation of Zimm plots, dn/dc analysis, reaction kinetics and polymer interaction analyses.

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