The TSKgel Protein A-5PW column has been designed for the rapid separation and robust quantification of a variety of antibodies. Monoclonal antibodies from harvested cell culture media can be captured and accurately quantitated in less than 2 minutes per injection. The column can be used for more than 2,000 injections without regeneration or cleaning.
Packed with hydroxylated methacrylic polymer beads, the TSKgel Protein A-5PW column is designed with a high degree of crosslinking, which allows high flow rate for chromatography while still maintaining chromatographic efficiency, peak width and resolution. The recombinant protein A ligand is a code-modified hexamer of the C domain. An enhanced rProtein A ligand is bound to the TSKgel 5PW base bead via multipoint attachment resulting in excellent base stability in 0.1 mol/L NaOH.
The wide range loading capacity of the TSKgel Protein A-5PW column can accurately determine the titer of mAb at various stages of cell culture media processing. The low level of protein A leaching makes this column a good candidate for small scale purification of mAbs for initial characterization.
|Pore size||100 nm|
|Particle size||20 µm|
|pH stability||2.5 – 12|
|Exclusion limit||1,000 kDa|
|Ligand||Recombinant protein A,
hexamer of c domain
|Ligand density||0.9 – 1.0 g/l gel|
|0023483||TSKgel Protein A-5PW||4.6 mm ID x 3.5 cm L|
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