TOYOPEARL polymeric AFC resins are available in a variety of group specific ligands. In addition, activated and reactive forms are available for the immobilization of target specific ligands. In AFC the mechanism of binding and elution varies depending on the immobilized ligand used. In some cases such as with TOYOPEARL AF-Chelate-650M (for Immobilized Metal Affinity Chromatography or IMAC) the binding and elution mechanism may change through the use of different metals or chemical modifiers. DBC values for AFC materials vary with ligand and are typically on the low side. However, because AFC has high selectivity and most of the capacity is used for the target, with feedstock impurities remaining unbound, AFC resins are typically used for the Capture and Intermediate purification steps of a manufacturing process.