Micro-PAC™ – Pillar Array Columns – a new generation of HPLC columns

Detect more molecules, with higher sensitivity, in tiny, complex biological samples

  • Micro-pillar array columns (µPAC™) deliver high peak capacity even at long gradient times, with high reproducibility, sensitivity, and precision.
  • They produce lower backpressure than conventionally packed columns.
  • They have excellent scalability for proteomic experiments and they deliver robust separations.
  • When used in combination with DIA methods, they enable single-shot comprehensive proteome analysis delivering more than 9,000 protein identifications in about six hours

Thanks to the unique pillar array technology, all columns ensure an optimal performance and an unprecedented reproducibility and robustness. They are compatible with all commercially available low flow LC systems, and can be integrated smoothly in your experimental set-up.  According to the specific demands of your separation challenge, configurations compatible with nano, capillary and microliter flow rate can be provided.

  • The current µPACᵀᴹ columns’ C18 stationary phase chemistry focuses on reversed phase HPLC for applications in proteomics, metabolomics, lipidomics and biopharmaceuticals. µPACᵀᴹ excels in conditions requiring excellent separation and sensitivity
  • Discovering new biomarkers is like finding needles in a haystack. Achieve more identifications from samples with a large dynamic range
  • Some compounds, like isomeric forms, are difficult to separate. Perform inter- and intra-class separation of human blood plasma lipids, resulting in high lipidome coverage
  • An in-depth characterization of new biologicals or biosimilars is an essential step in the approval process. Identify point mutations and post-translational modifications in monoclonal antibodies.

Depending on the sample complexity or the instrument time that can be dedicated to a certain sample, different LC columns and corresponding LC-MS/MS methods are required. We have created a complementary product portfolio addressing different needs. Find the µPACᵀᴹ product best suited for your challenges.


200 cm µPACᵀᴹ column
For a deep coverage of highly complex samples with long gradient times delivering the highest chromatographic performance.

50 cm µPACᵀᴹ column
For robust routine high-throughput proteomics workflows combining faster analyses with superior performance and improved reproducibility, even with limited sample amounts.

Capillary LC column

µPACᵀᴹ capLC column
For robust and highly sensitive analyses at capillary flow rates

Trapping column

µPACᵀᴹ Trapping column
Sample clean-up and preconcentration for improved column lifetime and workflow throughput.


µPACᵀᴹ-compatible EASY-SprayTM Emitter
Easily connect to Thermo Fisher EASY-Sprayᵀᴹ source-equipped mass spectrometers.

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